Withanolide A

Research use only, not for human use.

Withanolide A extends the lifespan in human EGFR-driven cancerous Caenorhabditis elegans.

Withanolide A extends the lifespan in human EGFR-driven cancerous Caenorhabditis elegans.

Cell Viability Assay Cell Line:CaOV3、SKOV3、OVCAR3、TOV112D and TOV21G Concentration:0, 0.078, 0.156, 0.313, 0.625, 1.25, 2.5, 5, 10 μM Incubation Time:72 h Result:Decreased the cell viability and with an IC50 concentrations at 520 nM, 627 nM, 452 nM, 262 nM, 243 nM, respectively.Apoptosis Analysis Cell Line:CaOV3, SKOV3 Concentration:0.5, 1, 2 μMIncubation Time:24, 48 h Result:Induced apoptosis in a dose-dependent manner.Cell Cycle Analysis Cell Line:CaOV3, SKOV3 Concentration:0.5, 1, 2 μM Incubation Time:24, 48 h Result:Induced G2/M phase cell cycle arrest.Western Blot Analysis Cell Line:CaOV3, SKOV3 Concentration:0.5, 1, 2 μM Incubation Time: 24, 48 h Result:Decreased cdc25C protein.Cyclin B1 stayed unchanged at 24 h but decreased by 48 h in a dose-dependent manner in CaOV3 cells, transiently increased in SKOV3 cells.Reduced the levels of phospho-Akt (ser 473) and Bcl-2.

Animal Model:Adult male Sprague Dawley rats weighing 240–250 g Dosage:10 μmol/kg Administration:p.o. (dissolved in 0.5% gum arabic solution)Result:Decreased the level of reactive oxygen species generation and lipid peroxidation.Increased the level of GSH and the activity of glutathione reductase, glutathione s transferase and superoxide dismutase.Increased the ATP, NADPH and GCLC activity.Decreased corticosterone level and glucocorticoid receptor.

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Angiogenesis

EGFR

EGFR|NF-κB|TNF-α

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32911-62-9

470.6

C28H38O6

>98% (HPLC)

In Vitro:?DMSO : 10 mg/mL (21.25 mM)

O=C1O[C@@]([H])([C@@](C)([C@H]2CC[C@]3([C@@]4([C@H]5[C@@H]([C@@](O)(CC=CC6=O)[C@]6([C@]4(CC[C@@]32C)[H])C)O5)[H])[H])O)CC(C)=C1C

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(-20℃)

With Ice Pack

≥ 2 years